colorectal cancer cell lines hct116  (ATCC)

Journal: Cancer Biology & Therapy

Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

doi: 10.1080/15384047.2026.2667568

Figure Lengend Snippet: Inhibition of MBD2 upregulated the expression of SFRP1 and inhibited Wnt signaling pathway activation. (A and B) The interference efficiency of MBD2 siRNAs at the protein level in SW480 and HCT116 cells. (C) Silencing MBD2 increased the mRNA level of SFRP1. (D and E) Silencing MBD2 increased the protein level of SFRP1 and decreased the protein level of β -catenin. (F) KCC07 disrupted MBD2 binding to the SFRP1 promoter. (G and H) The half inhibitory concentration (IC50) of KCC07 in CRC cells. (I) KCC07 increased the mRNA level of SFRP1. (J and K) KCC07 increased the protein level of SFRP1 and decreased the protein level of β -catenin. n = 6, *indicates p < 0.05, **indicates p < 0.01.

Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

Techniques: Inhibition, Expressing, Activation Assay, Binding Assay, Concentration Assay

Journal: Cancer Biology & Therapy

Article Title: MBD2 suppresses SFRP1 expression and promotes colorectal cancer development by blocking MED19 binding to its methylated promoter

doi: 10.1080/15384047.2026.2667568

Figure Lengend Snippet: The effect of KCC07 on recovering SFRP1 expression requires MED19. (A and B) The interfering efficiency of MED19 siRNAs at the protein level in SW480 and HCT116 cells. (C–F) Treatment with KCC07 resulted in increased SFRP1 expression and increased β -catenin expression at both the mRNA and protein levels in CRC cells, while simultaneous knockdown of MED19 abolished these effects. n = 6, ns indicates not significant, *indicates p < 0.05, and **indicates p < 0.01.

Article Snippet: Normal colon mucosa cell line NCM460 (ATCC, CRL-1642 TM ) and the human colorectal cancer cell lines HCT116 (ATCC, CCL-247 TM ) and SW480 (ATCC, CCL-228 TM ) were purchased from iCell, and all had STR identification reports.

Techniques: Expressing, Knockdown

Journal: Frontiers in Molecular Biosciences

Article Title: Bioinformatics combined with machine learning for the identification of malignant transformation markers in colorectal polyps

doi: 10.3389/fmolb.2026.1785464

Figure Lengend Snippet: scRNA-seq analysis of cell heterogeneity during colorectal carcinogenesis. (A) UMAP visualization of cell clusters from para-cancer, adenomatous polyp, and cancer tissues, and cancer tissues. A total of 15 independent cell clusters were identified. (B) Bubble plot of canonical marker gene expression for each cell clusters. (C) Heatmap showing the expression scores of the key WGCNA module genes across all identified cell clusters. Higher expression levels were predominantly observed in specific cell clusters, suggesting cell type-specific enrichment of these co-expression modules. (D–H) Violinplot showing the expression trends of module genes in corresponding cells.

Article Snippet: Human colorectal cancer cell lines (HCT116, LOVO, and RKO) were purchased from the ATCC, and the normal human colon mucosal epithelial cells (NCM460) was obtained from Yuchun Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Marker, Gene Expression, Expressing

Journal: Frontiers in Molecular Biosciences

Article Title: Bioinformatics combined with machine learning for the identification of malignant transformation markers in colorectal polyps

doi: 10.3389/fmolb.2026.1785464

Figure Lengend Snippet: Expression and survival analysis of malignant transformation markers. (A) Protein expression profiles of these genes in normal and colorectal tumor tissues were inferred using the UALCAN online platform. (B) Kaplan–Meier survival curves illustrating the correlation between gene expression levels and overall survival of CRC patients. The x-axis represents time in months, and the y-axis represents the percentage of surviving patients. The red line represents patients with high gene expression, while the black line represents patients with low gene expression.

Article Snippet: Human colorectal cancer cell lines (HCT116, LOVO, and RKO) were purchased from the ATCC, and the normal human colon mucosal epithelial cells (NCM460) was obtained from Yuchun Biotechnology Co., Ltd. (Shanghai, China).

Techniques: Expressing, Transformation Assay, Gene Expression